[Effects of Serum Containing Qinbai Qinfei Concentrated Pellets on Expressions of NLRP3 Inflammasome in RAW264.7 Cells Infected with Mycoplasma pneumonia].

Objective: To investigate the effects of serum containing Qinbai Qingfei concentrated pellets on expressions of NLRP3 inflammasome in RAW264. 7 cells infected with Mycoplasma pneumoniae( MP) IL-1ß. Methods: RAW264. 7 cells were randomly divided into normal group, MP model group and serum containing Qinbai Qinfei concentrated pellets group. RAW264. 7 cells and MP strain were cultured utilizing normal methods, preparation of serum containing Qinbai,with 1∶ 10 multiplicity of infection( MOI) of MP stimulation on RAW264. 7 cells; cells of each group were collected at 8,16,24 h respectively. The expressions of NLRP3,ASC and Caspase-1 mRNA were detected by the method of FQ-PCR. The expressions of NLRP3,ASC and Caspase-1 p20 protein were detected by Westernblot. The content of IL-1ß in the supernatant was measured by ELISA. Results: Compared with the normal group, he levels of NLRP3,ASC and Caspase-1 mRNA were significantly increased in the model group at 8,16,24 h respectively( P < 0. 05 or P < 0. 01); while the levels of NLRP3,ASC and Caspase-1 p20 protein were increased significantly( P < 0. 05 or P < 0. 01) at 16,24 h, and the levels of IL-1ß were increased at significantly( P < 0. 01) 24 h. Compared with the model group, the levels of NLRP3,ASC and Caspase-1 mRNA were significantly reduced in serum containing Qinbai Qinfei concentrated pellets group at 16,24 h( P < 0. 05 or P < 0. 01); the expressions of NLRP3,ASC,Caspase-1 p20 protein and the content of IL-1ß were all decreased at 24 h.Conclusion: The mechanism of antiMycoplasma pneumoniae action of Qinbai may be related to the down-regulation of NLRP3 inflammasome expressions.

Chemical constituents of Euonymus fortunei.

A new flavonol glycoside, kaempferol-3-O-ß-D-(2-O-E-p-coumaroyl)-glucopyranosyl-7-O-α-l-rhamnopyranoside (1), along with eleven known compounds including five flavonol glycosides (2-6), one phenolic glycoside (7), two megastigmane glycosides (8 and 9), two triterpenoids (10 and 11) and one alditol (12), was isolated from the aerial parts of Euonymus fortunei. Their structures were determined on the basis of spectroscopic analysis and chemical evidence. Compounds 2-4, 7, 8, and 10-12 were evaluated their antimicrobial activities against Ureaplasma urealyticumin vitro, but all tested compounds have no useful activities against Ureaplasma urealyticum.